FLUORESCENCE-FREE UV-VIS REFLECTION SPECTRA OF HUMAN SKIN

A fluorimeter operated in synchronous mode was used to record fluoresc ence-free UV/VIS reflection spectra of human skin. BaSO4 or Teflon ser ved as reflection standards. The variation coefficient of the spectral data showed a maximum around 350 nm. The relative remittance (normali zed spectra) increased dramatically after cyanoacrylate stripping arou nd 305 nm equivalent with loss of UV-absorption. Increase of moisture level by occlusion or washing of skin caused a similar but reversible change, washing with surfactants was provocing changes lasting for mor e than 24 hours. Exaggerated use of soap and surfactant bars drastical ly enlarged remittance around 300 nm.