Laser scanning confocal microscopy has been used to study the time evo
lution of a three dimensional spatially bicontinuous structure of a ph
ase-separated polymer blend undergoing the late stage of spinodal deco
mposition, as a model example of condensed matter systems. Bath the me
an and Gaussian curvatures of the interface between two coexisting pha
ses have been directly measured for the first time from the reconstruc
ted 3D image. The Gaussian curvature is negative, clearly demonstratin
g that the interface of the bicontinuous structure is anticlastic, whi
le the mean curvature is close to zero. Self-similar growth of the str
ucture is inferred from the time evolution of the average radius of cu
rvature of the interface.