H. Okamoto et al., INVITRO GRANULOMA-FORMATION BY SPLEEN-CELLS TREATED WITH PSORALEN PLUS LONG-WAVE ULTRAVIOLET-RADIATION, Photochemistry and photobiology, 57(4), 1993, pp. 667-669
In vitro granulomas are induced by culturing murine spleen cells with
artificial microparticles, dextran beads. In the presence of 0.5 mug/m
L 8-methoxypsoralen, UVA radiation (0.2-2.0 J/cm2) suppressed granulom
a formation in a UVA dose-dependent manner. The doses of PUVA did not
affect the cell viability as assessed by trypan blue exclusion. The ti
me course of granuloma formation in 0.5 J/cm2 PUVA-treated cells was s
imilar to that of normal spleen cells, with a maximum granuloma index
at day 3 of culture, although a 49-63% suppression of granuloma format
ion was observed. PUVA-treated, nonadherent cells produced the same gr
anuloma index when cultured with normal adherent cells. In contrast, a
smaller granuloma index was observed in PUVA-treated adherent cells e
ven when they were cultured with normal nonadherent cells. These data
suggest that PUVA alters macrophages, resulting in the suppression of
granuloma formation in vitro.