DEVELOPMENT OF A RAPID ASSIMILABLE ORGANIC-CARBON METHOD FOR WATER

A rapid method for measurement of assimilable organic carbon (AOC) is proposed. The time needed to perform the assay is reduced by increasin g the incubation temperature and increasing the inoculum density. The ATP luciferin-luciferase method quickly enumerates the test organisms without the need for plate count media or dilution bottles. There was no significant difference between AOC values determined with strain P1 7 for the ATP and plate count procedures. For strain NOX, the plate co unt procedure underestimated bacterial levels in some samples. Compari son of AOC values obtained by the Belleville laboratory (by the ATP te chnique) and the Stroud Water Research Center (by plate counts) showed that values were significantly correlated and not significantly diffe rent. The study concludes that the rapid AOC method can quickly determ ine the bacterial growth potential of water within 2 to 4 days.