EFFECT OF HUMAN CHORIONIC-GONADOTROPIN ADMINISTERED EARLY IN THE ESTROUS-CYCLE ON OVULATION AND SUBSEQUENT LUTEAL FUNCTION IN COWS

Cows of mixed beef breeds were randomly assigned to receive one of thr ee treatments: 1) i.v. injection of hCG on d 6 of the estrous cycle (e strus = d 0) and slaughter on d 12 to 14, 2) i.v. injection of saline on d 6 and slaughter on d 12 to 14, or 3) slaughter on d 5 to 7. Corpo ra lutea (CL) were collected at slaughter and weighed. A portion of ea ch CL was cultured for 6 h with no hormone or various doses of LH (25, 50, or 100 ng/mL of medium). All cows that received an hCG injection on d 6 (Treatment 1) exhibited a luteal structure in addition to the C L present at the time of hCG administration. Thus, the following CL we re evaluated: 6-d-old (induced) CL from Treatment 1 (d-6 hCG) and 13-d -old (natural) CL from Treatment 1 (d-13 hCG), 13-d-old CL from Treatm ent 2 (d 13), and 6-d-old CL from Treatment 3 (d 6). From d 9 to 13, d aily plasma progesterone concentrations were greater (P < .01) for cow s on Treatment 1 than for cows on Treatment 2. Whereas LH stimulated ( P < .01) progesterone secretion regardless of treatment, progesterone secretion in vitro was greater (P < .01) for CL from saline-treated co ws (Treatments 2 and 3) than for CL from hCG-treated cows (Treatment i ). No effect of hCG was observed for luteal DNA, RNA, or protein conce ntrations, or for luteal RNA:DNA or protein:DNA ratios. Across all tre atments, however, protein concentration was greater (P < .01) for d-13 than for d-6 CL, whereas RNA:DNA ratio was greater (P < .01) for d-6 than for d-13 CL. These data indicate that the large follicle present on d 6 is capable of ovulating in response to hCG and forming a functi onal CL.