THERMOSPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY (TSP LC MS) - ANALYSIS OF ACETYLATED APIGENIN-7-GLUCOSIDES FROM CHAMOMILLA-RECUTITA/

Extracts from the flowers of Chamomilla recutita have been examined fo r acetylated derivatives of the most abundant apigenin-7-O-beta-glucos ide (1). By using HPLC methods with photodiode-array detection and the rmospray liquid chromatography/mass spectrometry (TSP LC/MS), 4 isomer ic monoacetates have been found. Apart from the known 2''- and 6''-ace tates (2, 5) the 3''- and 4''-monoacetates (3, 4) were newly identifie d and separated by HPLC. In addition, a mixture of the 2'',3''- and 3' ',4''-diacetates (6, 7), which has been inseparable hitherto, has been resolved by HPLC. Except from apigenin-7-(6''-O-acetyl)-glucoside (5) all acetylated derivatives were found to undergo rapid ester hydrolys is.