OLIGOSACCHARIDE-BINDING MOLECULES OF THE SURFACE OF NEOPLASTIC HEMATOPOIETIC AND LYMPHOID HUMAN-CELLS

Using immunocytochemical methods and carbohydrates attached to biotiny lated polyacrylamide as the probes, the presence of oligosaccharide-bi nding molecules on the surface of normal and malignant hemopoietic and lymphoid cells were revealed. The specificity of the recognition was demonstrated by inhibition tests in the presence of the excess of low molecular weight ligands. An analysis of cell phenotype relation with the expression of carbohydrate-binding molecules was carried out. Prel iminary data based on the study of carbohydrate structures expression, show the differentiation of chronic B-lympholeukemia (low expression) from B-forms of non-Hodgkin's malignant lymphomas (high expression) a nd possibly ALL from AML also.