QUANTITATIVE-ANALYSIS OF CEFRADINE BY LIQUID-CHROMATOGRAPHY ON POLY(STYRENE-DIVINYLBENZENE)

Authors
HENDRIX C PIJCKE M YUN LM ROETS E HOOGMARTENS J
Citation
C. Hendrix et al., QUANTITATIVE-ANALYSIS OF CEFRADINE BY LIQUID-CHROMATOGRAPHY ON POLY(STYRENE-DIVINYLBENZENE), Journal of pharmaceutical and biomedical analysis, 11(2), 1993, pp. 145-155
Citations number
25
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
Journal of pharmaceutical and biomedical analysis → ACNP
ISSN journal
07317085
Volume
11
Issue
2
Year of publication
1993
Pages
145 - 155
Database
ISI
SICI code
0731-7085(1993)11:2<145:QOCBLO>2.0.ZU;2-F
Abstract
A method is described for isocratic analysis of cefradine by liquid ch romatography on a poly(styrene-divinylbenzene) column (PLRP-S, 250 X 4 .6 mm i.d.) at 50-degrees-C. Cefradine is separated from its related s ubstances using a mobile phase of acetonitrile-0.02 M sodium 1-octanes ulphonate-0.2 M phosphoric acid-water (14.5:10:5:up to 100, v/v/v/v). The flow rate was 1.0 ml min-1 and UV-detection was performed at 254 n m. The method was employed for the quantitative analysis of reference substances, bulk samples and pharmaceutical dosage forms.