A. Rosner et al., TRANSCRIPTION AMPLIFICATION OF POLYMERASE CHAIN-REACTION PRODUCTS OF BEAN YELLOW MOSAIC-VIRUS RNA EXTRACTED FROM GLADIOLI CORMS, Annals of Applied Biology, 121(2), 1992, pp. 269-276
The RNA genome of bean yellow mosaic virus (BYMV) was detected in glad
ioli corms by reverse transcription and polymerase chain reaction (PCR
). However, a positive signal was not obtained from some infected corm
s because the virus concentration was below the sensitivity limits of
PCR. Virus was detected in these corms by using a modified protocol in
which transcription of the PCR products by T7 RNA polymerase yielded
an additional 10- to 100-fold signal amplification. The relative conce
ntrations of BYMV in gladioli corms were evaluated using PCR. Virus ac
cumulation in corms was found to be a 100 times lower than in leaves,
and be within the range of picogram quantities of viral RNA per gram f
resh weight of corm tissue.