A novel approach to the quantitation of the muscular dystrophy protein
, dystrophin, in muscle extracts is described. The two-site ELISA uses
two monoclonal antibodies against dystrophin epitopes which lie close
together in the rod domain of the dystrophin molecule in order to min
imize the effects of dystrophin degradation. Dystrophin is assayed in
its native form by extracting with non-ionic detergents and avoiding t
he use of SDS.