A QUANTITATIVE ELISA FOR DYSTROPHIN

Citation
Ge. Morris et al., A QUANTITATIVE ELISA FOR DYSTROPHIN, Journal of immunological methods, 161(1), 1993, pp. 23-28
Citations number
18
Categorie Soggetti
Immunology
ISSN journal
00221759
Volume
161
Issue
1
Year of publication
1993
Pages
23 - 28
Database
ISI
SICI code
0022-1759(1993)161:1<23:AQEFD>2.0.ZU;2-#
Abstract
A novel approach to the quantitation of the muscular dystrophy protein , dystrophin, in muscle extracts is described. The two-site ELISA uses two monoclonal antibodies against dystrophin epitopes which lie close together in the rod domain of the dystrophin molecule in order to min imize the effects of dystrophin degradation. Dystrophin is assayed in its native form by extracting with non-ionic detergents and avoiding t he use of SDS.