MONOCLONAL ANTIBODY-SPECIFIC IMMOBILIZATION OF ERYTHROCYTE ANTIGENS (MAIEA) - A NEW TECHNIQUE TO SELECTIVELY DETERMINE ANTIGENIC SITES ON RED-CELL MEMBRANES
Ac. Petty, MONOCLONAL ANTIBODY-SPECIFIC IMMOBILIZATION OF ERYTHROCYTE ANTIGENS (MAIEA) - A NEW TECHNIQUE TO SELECTIVELY DETERMINE ANTIGENIC SITES ON RED-CELL MEMBRANES, Journal of immunological methods, 161(1), 1993, pp. 91-95
A new assay, the monoclonal antibody-immobilization of erythrocyte ant
igens (MAIEA) is described for the assignment of red cell antigens rec
ognised by human allo-antisera, to particular membrane components of t
he red cell membrane. This technique detects tri-molecular complexes f
ormed by the reaction of a human antibody and a mouse antibody with a
particular red cell protein. A positive reaction, in an ELISA-type det
ection procedure, occurs if the epitopes recognised by the human and m
ouse antibodies are present on the same membrane component but differe
nt regions. The MAIEA assay has been developed to investigate blood gr
oup antigens and in this report its application to the study of the Lu
theran, Yt and Kell blood group systems is described. The technique ha
s been used to show that the Lu(a) and Lu3 antigens are carried on the
Lutheran glycoprotein, yt(a) and yt(b) on the enzyme acetylcholineste
rase and K, k, Kp(a) and Kp(b) on the Kell glycoprotein.