Ja. Panza et al., ROLE OF ENDOTHELIUM-DERIVED NITRIC-OXIDE IN THE ABNORMAL ENDOTHELIUM-DEPENDENT VASCULAR RELAXATION OF PATIENTS WITH ESSENTIAL-HYPERTENSION, Circulation, 87(5), 1993, pp. 1468-1474
Background. Patients with essential hypertension have abnormal endothe
lium-dependent vasodilation. Because the endothelium exerts its action
on the vascular smooth muscle through the release of several substanc
es, it is important to identify which of these factors is involved in
the abnormal response of hypertensive arteries. Methods and Results. T
o investigate the role of endothelium-derived nitric oxide in this abn
ormality, we studied the vascular effect of the arginine analogue N(G)
-monomethyl-L-arginine, an inhibitor of the endothelial synthesis of n
itric oxide, under baseline conditions and during infusion of acetylch
oline, an endothelium-dependent vasodilator, and sodium nitroprusside,
a direct smooth muscle dilator. The study included 11 hypertensive pa
tients (seven men; age, 46.5+/-9 years) and 10 normal control subjects
(seven men; age, 45.7+/-7 years). Drugs were infused into the brachia
l artery, and the response of the forearm vasculature was measured by
strain-gauge plethysmography. Basal blood How was similar in normal co
ntrol subjects and hypertensive patients (2.97+/-0.7 versus 2.86+/-1.1
mL . min-1 . 100 mL-1, respectively). N(G)-monomethyl-L-arginine prod
uced a significantly greater decrease in blood flow in control subject
s than in patients (1.08+/-0.6 versus 0.32+/-0.4 mL . min-1 . 100 mL-1
; p < 0.004). The vasodilator response to acetylcholine was reduced in
patients compared with control subjects (maximum flow, 8.2+/-4 versus
16.4+/-8 mL . min-1 . 100 mL-1; p<0.001). N(G)-monomethyl-L-arginine
blunted the vasodilator response to acetylcholine in control subjects
(maximum flow decreased from 16.4+/-8 to 7.01+/-3 mL . min-1 . 100 mL-
1; p<0.004); however, the arginine analogue did not significantly alte
r the response to acetylcholine in hypertensive patients (maximum flow
, 8.2+/-4 versus 8.01+/-5 mL . min-1 . 100 mL-1). N(G)-monomethyl-L-ar
ginine did not modify the vasodilator response to sodium nitroprusside
in either control subjects or patients. Conclusions. These findings i
ndicate that patients with essential hypertension have a defect in the
endothelium-derived nitric oxide system that may at least partly acco
unt for both the increased vascular resistance under basal conditions
and the impaired response to endothelium-dependent vasodilators.