PURIFICATION AND PROPERTIES OF A CRUCIFORM DNA-BINDING PROTEIN FROM USTILAGO-MAYDIS

Authors
KOTANI H KMIEC EB HOLLOMAN WK
Citation
H. Kotani et al., PURIFICATION AND PROPERTIES OF A CRUCIFORM DNA-BINDING PROTEIN FROM USTILAGO-MAYDIS, Chromosoma, 102(5), 1993, pp. 348-354
Citations number
51
Categorie Soggetti
Genetics & Heredity
Journal title
Chromosoma → ACNP
ISSN journal
00095915
Volume
102
Issue
5
Year of publication
1993
Pages
348 - 354
Database
ISI
SICI code
0009-5915(1993)102:5<348:PAPOAC>2.0.ZU;2-Z
Abstract
A DNA binding protein with an M(r) of 1 1000 was purified from Ustilag o maydis. Its solubility in acid, amino acid composition, and mobility during gel electrophoresis are reminiscent of properties observed for the high mobility group nonhistone chromosomal proteins. The protein recognizes cruciform DNA made from oligonucleotides and also binds pre ferentially to a plasmid containing an extruded cruciform.