DETERMINATION OF INGENOL IN HOMEOPATHIC MOTHER TINCTURES OF EUPHORBIASPECIES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A high-performance liquid chromatograpic method was developed for the identification and determination of ingenol in homoeopathic mother tin ctures of eight Euphorbia species. The ingenol esters were hydrolysed with KOH in methanol and the free ingenol was measured using a C18 rev ersed-phase column with methanol-water (60:40, v/v) as the mobile phas e. The ingenol content ranged from 0.5 to 16.7 mug/ml. Euphorbia resin ifera Berg. mother tincture was found to be the richest in ingenol. On the other hand, the ingenol content was not significant in E. amygdal oides L. and E. pilulifera L. mother tinctures.