Ao. Converse et Jd. Optekar, A SYNERGISTIC KINETICS MODEL FOR ENZYMATIC CELLULOSE HYDROLYSIS COMPARED TO DEGREE-OF-SYNERGISM EXPERIMENTAL RESULTS, Biotechnology and bioengineering, 42(1), 1993, pp. 145-148
It is demonstrated that a two-enzyme component synergistic model can a
ccount for the observation that the degree of synergism goes through a
maximum as the total enzyme concentration is increased. The degree of
synergism is low at low enzyme concentration because the extent of co
nversion is low and therefore the cellulose chain ends, present origin
ally, are not exhausted; thus the action of the cellobiohydrolase (CBH
) is not dependent on the chain ends generated by the endoglucanase (E
G). The degree of synergism declines at high enzyme concentration due
to saturation of adsorption sites with CBH, thus decreasing the genera
tion of chain ends by EG.