H. Sato et al., INDUCTION OF A 23-KDA STRESS PROTEIN BY OXIDATIVE AND SULFHYDRYL-REACTIVE AGENTS IN MOUSE PERITONEAL-MACROPHAGES, Biochimica et biophysica acta, 1148(1), 1993, pp. 127-132
The synthesis of 23 kDa protein was enhanced when mouse peritoneal mac
rophages were exposed to oxidative agents such as hydrogen peroxide an
d menadione, or to sulfhydryl-reactive agents such as diethylmaleate,
cadmium chloride and sodium arsenite. After 11 h exposure to these age
nts the 23 kDa protein was one of the actively synthesized proteins in
the macrophages. Under similar conditions the 34 kDa protein previous
ly identified as heme oxygenase, was induced and its synthesis precede
d that of the 23 kDa protein. Neither the 23 kDa or the 34 kDa protein
was induced by hyperthermia. Conversely, the various oxidative and su
lfhydryl-reactive agents employed here did not induce the major heat s
hock proteins in the macrophages. When the macrophages were activated
by bacterial lipopolysaccharide or other stimulants, many proteins are
known to be induced, however, the 23 kDa and 34 kDa proteins were not
induced. The 34 kDa protein, i.e., heme oxygenase, has been found to
be stress-induced in various types of cell, but not the 23 kDa protein
. This suggests that the 23 kDa protein is a stress protein predominan
tly expressed in macrophages.