We have developed an interactive personal computer program for teachin
g the antinuclear antibody (ANA) test to medical technologists. The pr
ogram, called ANA-Tutor (Sanofi Diagnostics Pasteur Inc, Chaska, Minn)
, introduces the user to the microscope-based immunofluorescence assay
for ANA and examines understanding of the test. ANA-Tutor is based on
digital images of human epidermoid carcinoma cells that have been lab
eled with fluorescein-conjugated antibodies using a standard indirect
immunofluorescence assay for ANA. The program requires minimal compute
r literacy and has a number of advantages over traditional approaches
to teaching the ANA test.