HYBRID TANDEM MASS-SPECTROMETRY OF ELECTROSPRAY-PRODUCED PEPTIDE IONS

The collision-induced decomposition spectra of electrospray-produced m olecule ions were investigated on a hybrid tandem mass spectrometer. T he molecular mass of the peptides investigated ranged from m/z 409 (as the singly protonated molcule ion) to 1758 (as the singly, doubly and triply protonated molecules). The effect of changing the center-of-ma ss collision energy by changing either the laboratory collision energy or the mass of the target gas was investigated. The spectra of all io ns investigated showed a large number of sequence ions representative of the peptides' structures.