PURITY EVALUATION OF APROTININ BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A separation to the baseline isocratic technique has been developed fo r the evaluation of the purity of aprotinin by internal normalization. The column used was a 30 nm pore diameter butyl-bonded silica station ary phase. An exact relationship was found to give an adequate descrip tion of the retention of aprotinin using acetonitrile concentrations o f 17-22% and NaClO4 concentrations of 5-50 mmol. It can be used to opt imize the mobile phase content for the particular user column and to c ompensate for possible variations in the retention time of aprotinin, analysed on different batches of butyl-bonded stationary phases.