SENSITIVE DETERMINATION OF ENANTIOMERS OF AMINO-ACIDS DERIVATIZED WITH THE FLUOROGENIC REAGENT, 4-FLUORO-7-NITRO-2,1,3-BENZOXADIAZOLE, SEPARATED ON A PIRKLE-TYPE COLUMN, SUMICHIRAL OA 2500(S)

Some L- and D-amino acids (Phe or Leu) were derivatized with a fluorog enic reagent, 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and separa ted on a Pirkle-type column, Sumichiral OA 2500(S) [(S)-l-naphthylglyc yl-3,5-dinitrophenylamide silica gell with a mobile phase of 20 mm amm onium acetate in methanol. The fluorometric detection was made at 530 nm with excitation at 470 nm. No racemization of the enantiomers occur red during the derivatization reaction. The separation factors (a) for NBD-L-Phe and NBD-D-Phe, and NBD-L-Leu and NBD-D-Leu, were 1.27 and 1 .17, respectively. The detection limits were in the range of ca. 30 fm ol.