Antibodies raised against a purified fraction from microsomal membrane
s of leek epidermal cells were used to screen a lambdazap expression l
ibrary from epidermal cells of leek plants. A near full-length clone w
as isolated. This cDNA contains an open reading-frame of 1,191 bp codi
ng for a DnaJ protein (leek DNAJ 1 or LDJ1). Leek DnaJ1 represents the
second protein of this type described in a pluricellular organism, th
e first being that sequenced from human cells.