DEPLETION OF THE MONOAMINO METABOLITES OF ZOALENE DURING FROZEN STORAGE

3-Amino-5-nitro-o-toluamide and 5-amino-3-nitro-o-toluamide, the princ ipal metabolites in the tissues of chickens fed a diet containing Zoal ene (3,5-dinitro-o-toluamide), were shown to deplete in frozen liver t issues stored up to 1 year at -20-degrees-C, but not at -70-degrees-C. A slight loss of 5-amino-3-nitro-o-toluamide also occurred in thigh m uscle at -20-degrees-C. Evidence is presented that demonstrates that t he depletion of the metabolites was partially the result of their tran sformation to glucopyranosyl derivatives; both the alpha- and beta-ano mers of the 5-amino-3-nitro-o-toluamide conjugate were observed in liq uid chromatograms of tissue extracts.