IDENTIFICATION OF VEGETATIVE PROTEINS FOR A 2-DIMENSIONAL PROTEIN INDEX OF BACILLUS-SUBTILIS

Twenty-three of the most prominent spots which are visible on two-dime nsional (2-D) protein gels of Bacillus subtilis crude extracts were se lected as marker spots for the construction of a 2-D protein index. N- terminal sequencing of the corresponding proteins resulted in the iden tification of enzymes involved in glycolysis, TCA cycle, pentose phosp hate cycle, amino acid metabolism, nucleotide biosynthesis and transla tion. Using computer analysis of the 2-D protein gels, most of these m etabolic enzymes were found to be synthesized at a reduced rate after different stresses and glucose starvation. Such an approach permits a rapid and global evaluation of the regulation of different branches of metabolism in response to various physiological conditions.