AN INTRON CAPTURE STRATEGY USED TO IDENTIFY AND MAP A LYSYL OXIDASE-LIKE GENE ON CHROMOSOME-9 IN THE MOUSE

An intron capture strategy involving use of polymerase chain reaction was used to identify and map the mouse homologue of a human lysyl oxid ase-like gene (LOXL). Oligonucleotides complementary to conserved doma ins within exons 4 and 5 of the human lysyl oxidase-like gene were use d to amplify the corresponding segment from mouse genomic DNA. Sequenc ing of the resulting mouse DNA fragment of approximately 1 kb revealed that the exon sequences at the ends of the amplified fragment are hig hly homologous (90% nucleotide identity) to exons 4 and 5 of the human lysyl oxidase-like gene. An AluI restriction site polymorphism within intron 4 was used to map the mouse lysyl oxidase-like gene (Loxl) to mouse Chromosome 9 in a region that shares linkage conservation with h uman chromosome 15q24, to which the LOXL was recently mapped. (C) 1997 Academic Press.