PULSED UV LASER-INDUCED FLUORESCENCE DETECTION OF NATIVE PEPTIDES ANDPROTEINS IN CAPILLARY ELECTROPHORESIS

Authors
CHAN KC JANINI GM MUSCHIK GM ISSAQ HJ
Citation
Kc. Chan et al., PULSED UV LASER-INDUCED FLUORESCENCE DETECTION OF NATIVE PEPTIDES ANDPROTEINS IN CAPILLARY ELECTROPHORESIS, Journal of liquid chromatography, 16(9-10), 1993, pp. 1877-1890
Citations number
14
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of liquid chromatography → ACNP
ISSN journal
01483919
Volume
16
Issue
9-10
Year of publication
1993
Pages
1877 - 1890
Database
ISI
SICI code
0148-3919(1993)16:9-10<1877:PULFDO>2.0.ZU;2-5
Abstract
A pulsed UV laser operating at 248 nm was used for the laser-induced f luorescence (LIF) detection of native tryptophan-containing compounds in capillary electrophoresis. The limit of detection (LOD) of tryptoph an was found to be 3.3x10(-9) M (S/N=2). The LODs of the model protein s conalbumin and bovine serum albumin were found to be 1.3x10(-9) and 4x10(-9) M (S/N=2), respectively. These results were at least two orde rs of magnitude more sensitive when compared to UV absorption at 214 n m.