EGG-WHITE PROTEIN SEPARATION DEVELOPMENT - EFFECT OF BUFFERS AND UNTREATED FUSED-SILICA VS NEUTRAL COVALENTLY BONDED COLUMNS

A method for the separation of three egg white proteins, lysozyme. con albumin, and ovalbumin, was developed based on desired criteria of rep roducible baseline separation of all components in under 25 minutes. F ixed parameters were 50cm effective column length, 200 volts/cm field strength. Sodium phosphate, sodium borate. and phosphate/borate combin ation buffers were compared in different ionic strengths. The performa nce of bonded phase columns, CElect(Tm)-P1 and CElect-H (patent pendin g) were compared to that of untreated fused silica. The optimal separa tion was obtained using 100mM phosphate / 60 mM borate buffer, pH 8.0 on the hydrophilic CElect-P1 bonded phase column.