CONFIRMATORY TESTING OF CHLAMYDIA-TRACHOMATIS SYVA ENZYME-IMMUNOASSAYGRAY ZONE SPECIMENS BY SYVA DIRECT FLUORESCENT-ANTIBODY TEST

A series of gray zone specimens with sample to cutoff ratio of 0.7 to 0.99 encountered in routine use of the Syva Microtrak (Syva, Inc.) Chl amydia trachomatis enzyme immunoassay (EIA) test for urogenital specim ens were subjected to repeat testing in duplicate and high-speed centr ifugation with direct fluorescent antibody testing of the centrifugate . Immunofluorescent C. trachomatis elementary bodies were observed in high-speed centrifugates in more than 40% of two series of gray zone s pecimens examined indicating that a C. trachomatis gray zone result wo uld require confirmatory testing.