Ds. Peeper et A. Zantema, ADENOVIRUS-E1A PROTEINS TRANSFORM CELLS BY SEQUESTERING REGULATORY PROTEINS, Molecular biology reports, 17(3), 1993, pp. 197-207
Cell transformation by adenovirus-E1A proteins is mediated by binding
to cellular proteins whose functions are thereby inactivated or altere
d. The various properties of the E1A proteins are reviewed in relation
to their binding to cellular proteins. A number of the cellular prote
ins which associate to E1A have been identified: the retinoblastoma-su
sceptibility protein (Rb), the p107 protein, cyclin A and the p33cdk2
kinase. Recent data have shown that those proteins are also able to bi
nd to transcription factor E2F. Binding of Rb to E2F represses the tra
nscription-activating potential of E2F. E1A can sequester the regulato
ry proteins, like Rb, and thereby release free, active E2F. The domain
s in E1A that are essential for this transcriptional regulation are al
so required for the transforming properties of E1A.