Xh. Chen et al., SOLID-PHASE EXTRACTION OF MORPHINE FROM WHOLE-BLOOD BY MEANS OF BOND ELUT CERTIFY COLUMNS, Journal of forensic sciences, 38(3), 1993, pp. 668-676
The use of Bond Elut Certify columns for the isolation of morphine fro
m whole blood was evaluated. In order to monitor possible losses and t
he elution profile of morphine, a small amount of the tritiated analog
ue was added to the samples. Four sample pretreatment methods, three p
rotein precipitation methods and one sonication/dilution method, were
tested. The latter one gave the best results. The blood sample was app
lied onto the column at pH 3.3 after sonication and dilution with 0.1
M phosphate buffer (pH 3.3). The retention of morphine was affected by
the pH of the samples, and the loss of morphine during sample applica
tion was minimized at low pH (3.3). The interferences were removed by
washing the column with the phosphate buffer, 0.01 M acetic acid (pH 3
.3), and methanol, sequentially. Ammoniated methanol, 2 mL at 2%, was
selected to clute morphine. As a result, more than 80% of H-3-morphine
was recovered for concentrations of morphine ranging from 5 to 4000 n
g/mL.