SUBZERO TEMPERATURE OPERATING BIOSENSOR UTILIZING AN ORGANIC-SOLVENT AND QUINOPROTEIN GLUCOSE-DEHYDROGENASE

Authors
SODE K NAKASONO S TANAKA M MATSUNAGA T
Citation
K. Sode et al., SUBZERO TEMPERATURE OPERATING BIOSENSOR UTILIZING AN ORGANIC-SOLVENT AND QUINOPROTEIN GLUCOSE-DEHYDROGENASE, Biotechnology and bioengineering, 42(2), 1993, pp. 251-254
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology and bioengineering → ACNP
ISSN journal
00063592
Volume
42
Issue
2
Year of publication
1993
Pages
251 - 254
Database
ISI
SICI code
0006-3592(1993)42:2<251:STOBUA>2.0.ZU;2-P
Abstract
A subzero temperature operating biosensor was constructed using immobi lized quinoprotein glucose dehydrogenase (PQQGDH), glassy carbon elect rode, soluble electron mediator (ferrocene monocarboxylic acid), and a n organic solvent, ethylene glycol, as an antifreezing reagent. Using this biosensor, glucose concentration can be determined even at -7-deg rees-C. At this temperature, the response was 20% of that obtained at 20-degrees-C. This is the first study describing a subzero temperature operating biosensor.