THE YAL017 GENE ON THE LEFT ARM OF CHROMOSOME-I OF SACCHAROMYCES-CEREVISIAE ENCODES A PUTATIVE SERINE THREONINE PROTEIN-KINASE

The DNA sequence of a region between the LTE1 and CYS3 genes on the le ft arm of chromosome I from Saccharomyces cerevisiae contains an open reading frame (ORF), YAL017, corresponding to the 5.0 kb FUN31 (Functi on Unknown Now) transcribed region. The predicted protein from this OR F contains 1358 amino acid residues with a molecular weight of 152 531 , and an identifiable serine/threonine protein kinase catalytic domain . When compared with other yeast protein kinases, the Ya1017p kinase m ost resembles the SNF1 serine/threonine protein kinase which is involv ed in regulating sucrose fermentation genes. The Ya1017p kinase shows highest amino acid identities with two mammalian carcinoma-related ser ine/threonine protein kinases; PIM-1, which shows induced expression i n T-cell lymphomas; and p78A1, whose expression is lost in human pancr eatic carcinomas. Gene disruption of YAL017 indicates that it is non-e ssential for growth on glucose.