EFFECT OF SPACER GROUPS CONNECTING INSULIN WITH FLUOROPHORE IN GLY(A1)-SULFOBENZOXADIAZOLE-LABELED INSULINS ON THE IMMUNOREACTIVITY TOWARD ANTIINSULIN ANTIBODY

The immunoreactivity (specific binding) of three Gly(A1)-sulfobenzoxad iazole-labeled insulins which have different spacer groups, (4-sulfobe nzoxadiazol-7-ylthio)propanoyl]-insulin, [2-(4-sulfobenzoxadiazol-7-yl thio)acetyl]-insulin, Gly(Al)-[3-carboxy-2(or (4-sulfobenzoxadiazol-7- ylthio)propanoyl]-insulin, toward an anti-porcine insulin monoclonal a ntibody was investigated, where solid-phase fluoroimmunoassay techniqu e was utilized. The immunoreactivities of three labeled insulins were 2.1, 1.8 and 1 in that order.