A POTASSIUM CHANNEL TOXIN FROM THE SECRETION OF THE SEA-ANEMONE BUNODOSOMA-GRANULIFERA - ISOLATION, AMINO-ACID-SEQUENCE AND BIOLOGICAL-ACTIVITY

Authors
ANEIROS A GARCIA I MARTINEZ JR HARVEY AL ANDERSON AJ MARSHALL DL ENGSTROM A HELLMAN U KARLSSON E
Citation
A. Aneiros et al., A POTASSIUM CHANNEL TOXIN FROM THE SECRETION OF THE SEA-ANEMONE BUNODOSOMA-GRANULIFERA - ISOLATION, AMINO-ACID-SEQUENCE AND BIOLOGICAL-ACTIVITY, Biochimica et biophysica acta, 1157(1), 1993, pp. 86-92
Citations number
37
Categorie Soggetti
Biophysics,Biology
Journal title
Biochimica et biophysica acta → ACNP
ISSN journal
00063002
Volume
1157
Issue
1
Year of publication
1993
Pages
86 - 92
Database
ISI
SICI code
0006-3002(1993)1157:1<86:APCTFT>2.0.ZU;2-R
Abstract
A peptide toxin affecting potassium channels was isolated from the sea anemone Bunodosoma granulifera. It facilitates acetylcholine release at avian neuromuscular junctions, competes with dendrotoxin 1, a probe for voltage-dependent potassium channels, for binding to synaptosomal membranes of rat brain with a K(i) of 0.7 nM and suppresses K+ curren ts in rat dorsal root ganglion neurones in culture. It represents a ne w structural type of potassium channel toxin with the sequence V1RCDWF KETA10CRHAKSLGNC20RTSQKYRANC30AKTLQCC37 (M(r) 4275, three disulfides).