HPLC DETERMINATION OF INORGANIC CATION LEVELS IN CSF AND PLASMA OF CONSCIOUS RABBITS

Authors
FROSINI M GORELLI B MATTEINI M PALMI M VALOTI M SGARAGLI GP
Citation
M. Frosini et al., HPLC DETERMINATION OF INORGANIC CATION LEVELS IN CSF AND PLASMA OF CONSCIOUS RABBITS, Journal of pharmacological and toxicological methods, 29(2), 1993, pp. 99-104
Citations number
10
Categorie Soggetti
Toxicology,"Pharmacology & Pharmacy
Journal title
Journal of pharmacological and toxicological methods → ACNP
ISSN journal
10568719
Volume
29
Issue
2
Year of publication
1993
Pages
99 - 104
Database
ISI
SICI code
1056-8719(1993)29:2<99:HDOICL>2.0.ZU;2-U
Abstract
An HPLC method is described using conductimetric detection for the qua ntitative determination of sodium, potassium, magnesium, and calcium i n cerebrospinal fluid (CSF) and plasma of conscious rabbits. This meth od enabled the four cations to be estimated with rapidity, sensitivity , accuracy, and precision. The mean millimolar concentrations +/- SD f ound in CSF and (plasma) of 15 untreated animals were as follows: sodi um, 146.96 +/- 17.84 (135.06 +/- 20.11); potassium, 3.32 +/- 0.56 (4.5 7 +/- 1.03); magnesium, 0.90 +/- 0.20 (0.72 +/- 0.13); and calcium, 1. 47 +/- 0.19 (3.32 +/- 0.59).