DETECTION OF SALMONELLA IN HUMAN FECES BY RAMBACH AGAR AND BY A COLORLATEX AGGLUTINATION ASSAY

Authors
HEIZMANN WR
Citation
Wr. Heizmann, DETECTION OF SALMONELLA IN HUMAN FECES BY RAMBACH AGAR AND BY A COLORLATEX AGGLUTINATION ASSAY, Medical microbiology letters, 2(3), 1993, pp. 131-137
Citations number
NO
Categorie Soggetti
Microbiology
Journal title
Medical microbiology letters → ACNP
ISSN journal
10184627
Volume
2
Issue
3
Year of publication
1993
Pages
131 - 137
Database
ISI
SICI code
1018-4627(1993)2:3<131:DOSIHF>2.0.ZU;2-H
Abstract
The most sensitive method for detection of 100 Salmonella strains in 2 70 human faeces proved to be enrichment in selenite broth with subsequ ent subculture on solid media (100%), followed by direct plating on Sa lmonella Shigella agar (85%), Rambach agar (68%), and latex agglutinat ion after enrichment in selenite broth (64%). The detection limit of t he latex procedure for Salmonella enteritidis could be estimated to be between 1x10(7) to 1x10(8) CFU/ml. Co-culture with Escherichia coli s uppressed growth of Salmonella enteritidis significantly.