PURIFIED HEMOGLOBIN PREPARATIONS IN THE EVALUATION OF HBA1C DETERMINATION BY ION-EXCHANGE CHROMATOGRAPHY

The use of ion exchange resins for the estimation of HbA1c in clinical samples rests on the assumption that HbA1c is effectively and efficie ntly separated from other N-terminally modified haemoglobins and from HbA(o). To test this assumption, we applied highly purified preparatio ns of HbA1a+1b, HbA1c and HbA(o) to ion exchange minicolumns, using co nditions of application simulating actual blood samples and the first and second elution buffers provided by the manufacturer. The authentic ity and purity of the applied haemoglobin preparations were documented by high performance liquid chromatography, gel electrophoresis and ca rbohydrate content. About 40% of the applied HbA1a+1b eluted in the fi rst fraction; 45% eluted in the second fraction, and 10% to 15% requir ed 1 mol/L NaCl to elute from the column. Of the applied HbA1c, 65-80% eluted where expected in the second fraction, about 20% required 1 mo l/L NaCl to elute from the column, and the remainder eluted with HbA1a +1b. Some 3-6% of pure HbA(o) applied to minicolumns emerged in the se cond fraction, with the remainder eluting as expected after making the buffer 1 mol/L in NaCl. The results indicate that the fraction elutin g from ion exchange minicolumn chromatography that is designated 'HbA1 c' contains HbA1a+1b, and that a substantial portion of the HbA1c in a n applied sample does not elute in this fraction.