DECREASES IN THE SIZE AND PROLIFERATION RATE OF VIP-IMMUNOREACTIVE CELLS INDUCED IN-VITRO BY TESTOSTERONE ARE ASSOCIATED WITH DECREASES IN VIP RELEASE

Authors
CARRETERO J RUBIO M VAZQUEZPERFECTO RJ SANCHEZ F TORRES JL PEREZ RM VAZQUEZ R
Citation
J. Carretero et al., DECREASES IN THE SIZE AND PROLIFERATION RATE OF VIP-IMMUNOREACTIVE CELLS INDUCED IN-VITRO BY TESTOSTERONE ARE ASSOCIATED WITH DECREASES IN VIP RELEASE, Neuroendocrinology, 65(3), 1997, pp. 173-178
Citations number
29
Categorie Soggetti
Neurosciences,"Endocrynology & Metabolism
Journal title
Neuroendocrinology → ACNP
ISSN journal
00283835
Volume
65
Issue
3
Year of publication
1997
Pages
173 - 178
Database
ISI
SICI code
0028-3835(1997)65:3<173:DITSAP>2.0.ZU;2-4
Abstract
An in vitro study was carried out to determine whether testosterone di rectly inhibits the secretion of pituitary vasoactive intestinal pepti de (VIP). A study was also conducted to elucidate the repercussions of treatment with 10(-6) M testosterone of monolayer cultures in the siz e and proliferation rate of pituitary VIP-immunoreactive cells, using double immunocytochemical labelling for proliferating cell nuclear ant igen (PCNA) and VIP. Testosterone decreased the in vitro release of VI P from pituitary cells. It also decreased the percentage of pituitary VIP-immunoreactive and PCNA- and VIP-immunoreactive cells and decrease d the size of the cellular and nuclear areas of these cells. Our resul ts suggest that the inhibition of pituitary VIP secretion induced by t estosterone could be exerted at pituitary level and that testosterone could act as a modulator of the proliferation rate of rat pituitary VI P-immunoreactive cells.