ANALYSIS OF X-GAL PLATES FOR QUANTITATIVE PLASMID STABILITY DETERMINATIONS IN SACCHAROMYCES-CEREVISIAE

Authors
WANG Z DASILVA NA
Citation
Z. Wang et Na. Dasilva, ANALYSIS OF X-GAL PLATES FOR QUANTITATIVE PLASMID STABILITY DETERMINATIONS IN SACCHAROMYCES-CEREVISIAE, Biotechnology techniques, 7(5), 1993, pp. 325-328
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
7
Issue
5
Year of publication
1993
Pages
325 - 328
Database
ISI
SICI code
0951-208X(1993)7:5<325:AOXPFQ>2.0.ZU;2-Q
Abstract
X-gal plates were evaluated for the measurement of plasmid stability i n yeast cells carrying the Escherichia coli lacZ gene. The influence o f the medium, cloned gene number, and cell state on the accuracy of th e results was determined by comparison with traditional replica platin g. With few exceptions, X-gal plates provide a good quantitative one-s tep assay for plasmid stability in Saccharomyces cerevisiae expressing beta-galactosidase.