TRANSFORMATION OF RIFAMYCIN-B WITH IMMOBILIZED RIFAMYCIN OXIDASE OF CURVULARIA-LUNATA

Authors
BANERJEE UC
Citation
Uc. Banerjee, TRANSFORMATION OF RIFAMYCIN-B WITH IMMOBILIZED RIFAMYCIN OXIDASE OF CURVULARIA-LUNATA, Biotechnology techniques, 7(5), 1993, pp. 339-345
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
7
Issue
5
Year of publication
1993
Pages
339 - 345
Database
ISI
SICI code
0951-208X(1993)7:5<339:TORWIR>2.0.ZU;2-G
Abstract
Rifamycin oxidase of Curvularia lunata was immobilized on polyacrylami de gel. The optimum pH and temperature for immobilized enzyme reaction were 6.5 and 50-degrees-C, respectively. Enzyme stability increased o n immobilization and the half lives of immobilized enzyme preparations at 30 and 40-degrees-C were 30 and 11.5 d, respectively. With 2.5 mm beads diffusional resistances were observed. Reusability studies showe d that 1 mm size beads gave a higher rate of transformation in compari son with 2 or 2.5 mm beads.