RAPID, ACCURATE AND SENSITIVE DETERMINATION OF MOLECULAR-WEIGHT OF N-15 AND C-13 LABELED RECOMBINANT PROTEINS BY ELECTROSPRAY IONIZATION MASS-SPECTROMETRY

Authors
HEATH TG THANABAL V YE QZ
Citation
Tg. Heath et al., RAPID, ACCURATE AND SENSITIVE DETERMINATION OF MOLECULAR-WEIGHT OF N-15 AND C-13 LABELED RECOMBINANT PROTEINS BY ELECTROSPRAY IONIZATION MASS-SPECTROMETRY, Biotechnology techniques, 7(5), 1993, pp. 367-372
Citations number
8
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
7
Issue
5
Year of publication
1993
Pages
367 - 372
Database
ISI
SICI code
0951-208X(1993)7:5<367:RAASDO>2.0.ZU;2-M
Abstract
Electrospray ionization mass spectrometry (ES-MS) was utilized to accu rately measure the molecular weight of uniformly N-15 and C-13/N-15 is otope labeled stromelysin catalytic domain (20 kDa), which in turn was used to determine the degree of isotope enrichment in the protein.