ADH AND ADH-DUP SEQUENCES OF DROSOPHILA-LEBANONENSIS AND D-IMMIGRANS - INTERSPECIES COMPARISONS

We have cloned and sequenced the Adh genomic region of Drosophila leba nonensis (subgenus Scaptodrosophila) and D. immigrans (subgenus Drosop hila). This region, which contains Adh, encoding the alcohol dehydroge nase enzyme, and Adh-dup (duplicate of Adh), has been compared with th e same fragment from D. subobscura (subgenus Sophophora). Even though the flanking regions and introns of both genes have been affected by h igh substitution rates, the consensus sequences have been clearly iden tified. Although the overall homology of the coding regions was 76-78% among the species compared, there were differences in the exon distri bution of the nucleotide substitutions when Adh or Adh-dup were compar ed, thus showing that these two genes differ in their evolutionary pat tern.