The enhanced recovery of residual oil from unconsolidated carbonate co
res using microorganisms was examined in model cores packed with chips
of Viola limestone. Vibrio aspartigenicus strain GSP-1, an acid produ
cer, and Bacillus licheniformis strain JF-2, a surfactant producer, we
re used as the test microorganisms. The primary hypothesis tested was
that microbial treatment of a packed core system would result in the r
ecovery of residual oil from the carbonate test system. After three tr
eatments, 39-44% of the residual oil-in-place was recovered from packe
d core systems after treatment with strain GSP-1. Two treatments with
strain JF-2 resulted in a 27% recovery of the residual oil-in-place. M
odification of the core matrix was indicated by the presence of calciu
m in the core effluents and significant amounts of carbonate fines fou
nd in the dissected cores, presumably caused by dissolution of the car
bonate matrix by microbial acid end products of metabolism (microbial
matrix acidizing). These results are encouraging since significant amo
unts of crude oil were recovered in these systems with only a few trea
tments, suggesting that microbially enhanced oil recovery (MEOR) in ca
rbonate petroleum reservoirs could be efficient and economical.