GASTRIC AND PANCREATIC LIPASE LEVELS DURING A TEST MEAL IN DOGS

The levels of gastric and pancreatic lipases in the duodenum and the j ejunum were measured during the digestion of a test meal in dogs. Usin g both a specific enzymatic titration and an enzyme-linked immunosorbe nt assay, it is shown for the first time that gastric lipase remains a ctive in the duodenal and jejunal contents. An experimental device was set up for measuring the secretions and the intestinal flows of lipas es during the digestion of a liquid test meal. In a dog equipped with gastric and duodenal cannulae, the secretion of gastric lipase was sti mulated by food ingestion, reaching 3.0 +/- 0.3 mg/h (three times the basal secretion rate) during the lst h of digestion. The total secreto ry outputs of gastric and pancreatic lipases recorded over a 3-h perio d of digestion were 7.2 +/- 1.2 mg and 18.7 +/- 1.2 mg, respectively.