COMPARISON OF LOW-ENERGY AND HIGH-ENERGY COLLISION-INDUCED DISSOCIATION TANDEM MASS-SPECTROMETRY IN THE ANALYSIS OF RICINOLEIC AND RICINELAIDIC ACID

Citation
L. Nizigiyimana et al., COMPARISON OF LOW-ENERGY AND HIGH-ENERGY COLLISION-INDUCED DISSOCIATION TANDEM MASS-SPECTROMETRY IN THE ANALYSIS OF RICINOLEIC AND RICINELAIDIC ACID, Journal of mass spectrometry., 32(3), 1997, pp. 277-286
Citations number
24
Categorie Soggetti
Chemistry Inorganic & Nuclear",Spectroscopy,Biophysics
ISSN journal
10765174
Volume
32
Issue
3
Year of publication
1997
Pages
277 - 286
Database
ISI
SICI code
1076-5174(1997)32:3<277:COLAHC>2.0.ZU;2-4
Abstract
Ricinoleic acid and its trans isomer ricinelaidic acid, and their meth yl esters, were analyzed by low- and high-energy collision-induced dis sociation (CID) tandem mass spectrometry. It is shown that a stable ch arge centre is required to observe the rearrangement, which occurs in the beta-hydroxyalkene part and results in the loss of heptaldehyde, a nd that this rearrangement corresponds to a low-energy CID reaction. D ifferences between the CID behaviour of ricinoleic acid and that of it s trans isomer are related to the loss of water, which can also be reg arded as a low-energy rearrangement reaction. Comparison of low- and h igh-energy CID spectra further revealed that high-energy CID gives ris e to loss of a H-. radical and H-2 together with low-energy fragmentat ion. Examination of different molecule ions, including [M - H](-), [M + Li](+) and [M - H + 2Li](+) ions of free fatty acids and [M + Li] ions of the methyl esters shows that charge-remote homolytic fragmenta tion is most pronounced for the [M + Li](+) ions of the methyl esters. (C) 1997 by John Whey & Sons, Ltd.