A METASTATIC MODEL OF HUMAN COLON CANCER CONSTRUCTED USING CECAL IMPLANTATION OF CANCER-TISSUE IN NUDE-MICE

COL-2-JCK, a human colon cancer xenograft line able to be transplanted into nude mice, was implanted in the subserosal layer of the cecum, e ither as cancer tissue or as a single cell suspension. When cancer tis sue was used for the cecal implantation, 100% extensive local tumor gr owth and a high incidence of metastases to the regional lymph nodes, p eritoneum, liver, and lung was observed. In contrast, when the cell su spension of this line was injected into the cecal wall, no metastases were observed, with significantly reduced local tumor growth. The use of cancer tissue maintaining the original cancer tissue structure is t herefore considered imperative for allowing full expression of the bio logical characteristics of cancer cells. This nude mouse model using t he cecal implantation of cancer tissue should thus prompt further stud y on the biology of human colon cancer.