ANTIBACTERIAL EFFICACY OF NEW COMMERCIALLY MANUFACTURED DISINFECTANT SUBSTANCES AGAINST SALMONELLA-TYPHIMURIUM

The antibacterial effect of 19 new commercially manufactured disinfect ant substances on a Salmonella typhimurium strain was studied. The sub stances tested represent 9 quaternary ammonium salts (QAT) and 11 comb inated QAT with other ingredients. The antimicrobial efficacy was char acterized by influencing the growth and reproduction of bacterial cell s expressed either by MIC and ED(50) values (ED(50) values represent c oncentration of substance in mu g/ml which cause inhibition of growth by 50%), as well as by the inhibition of incorporation rate of [C-14] adenine and [C-14] leucine. The disinfectants are divided into three g roups according to their efficacy. The first group comprises substance s with strong inhibitory effect (MIG 0.04-0.19 mu g/ml) such as Neoqua t S, Antibacteric P, Divoquat forte, Sokrena and Diesin forte (sole fr om the group belonging to multicomponent substances). QAT except Antib acteric P interfere with energy metabolism (R values similar to 1). Th e second group represents substances with good antibacterial efficacy (MIG values up 1.56 mu g/ml), and the third group substances with MIC values up 12.5 mu g/ml. Cutasept G was found ineffective also in the c oncentration 100 mu g/ml. The method of inhibition of [C-14] precursor s is suitable as one from possible criterion in evaluation of antibact erial efficacy of various synthetic substances.