DISTRIBUTION OF RYANODINE RECEPTOR-LIKE IMMUNOREACTIVITY IN MAMMALIANCENTRAL-NERVOUS-SYSTEM IS CONSISTENT WITH ITS ROLE IN CALCIUM-INDUCEDCALCIUM RELEASE
P. Sah et al., DISTRIBUTION OF RYANODINE RECEPTOR-LIKE IMMUNOREACTIVITY IN MAMMALIANCENTRAL-NERVOUS-SYSTEM IS CONSISTENT WITH ITS ROLE IN CALCIUM-INDUCEDCALCIUM RELEASE, Neuroscience, 54(1), 1993, pp. 157-165
The distributions of ryanodine receptor-like immunoreactivity and Ca A
TPase-like immunoreactivity were identified in the guinea-pig and rat
central nervous system using antibodies raised against the rabbit skel
etal muscle ryanodine receptor and Ca ATPase. In both guinea-pig and r
at cerebellum, the ryanodine receptor-like immunoreactivity was restri
cted to the soma and dendrites of Purkinje cells. In the medulla, neur
on somata in the hypoglossal nucleus were stained in both species, but
in the dorsal motor nucleus of the vagus somata were stained in guine
a-pigs but not in rats. This species difference in ryanodine receptor-
like immunoreactivity is consistent with the species difference in exp
ression of a ryanodine sensitive, calcium activated potassium conducta
nce in neurons of the dorsal motor nucleus of the vagus. Immunoreactiv
ity to Ca- ATPase was present in vagal motoneurons in both species wit
h denser staining in the guinea-pig. The data further support the idea
that. in neurons of the dorsal motor nucleus of the vagus, release of
intracellular calcium stores via a ryanodine receptor activates a spe
cific class of potassium channels, thereby modulating cell exicitabili
ty.