SEQUENCES OF GENES ENCODING NAPHTHALENE DIOXYGENASE IN PSEUDOMONAS-PUTIDA STRAINS G7 AND NCIB-9816-4

Authors
SIMON MJ OSSLUND TD SAUNDERS R ENSLEY BD SUGGS S HARCOURT A SUEN WC CRUDEN DL GIBSON DT ZYLSTRA GJ
Citation
Mj. Simon et al., SEQUENCES OF GENES ENCODING NAPHTHALENE DIOXYGENASE IN PSEUDOMONAS-PUTIDA STRAINS G7 AND NCIB-9816-4, Gene, 127(1), 1993, pp. 31-37
Citations number
25
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
127
Issue
1
Year of publication
1993
Pages
31 - 37
Database
ISI
SICI code
0378-1119(1993)127:1<31:SOGEND>2.0.ZU;2-X
Abstract
The multicomponent enzyme, naphthalene dioxygenase, initiates the meta bolism of naphthalene by Pseudomonas putida strains G7 (PpG7) and NCIB 9816-4 (Pp9816-4). The genes involved (nahAaAbAcAd) are encoded by th e NAH7 and pDTG1 plasmids, respectively, and form part of the nah oper on. The locations of the structural genes were determined on previousl y cloned fragments of DNA. The nucleotide (nt) sequences were determin ed for nahAaAb from Pp9816-4 and for nahAaAbAcAd from PpG7. The approp riate open reading frames were identified using N-terminal amino acid sequences determined from the purified proteins. The two nt sequences showed 93% homology, with the least homology seen upstream from the pr omoter region.