CONTINUOUS INVITRO MULTIPLICATION OF SHOOT BUDS OF NORWAY SPRUCE (PICEA-ABIES L) BY INTERMITTENT APPLICATION OF GROWTH-REGULATORS

Authors
KUNZE I GRAFE R SCHIEMANN J
Citation
I. Kunze et al., CONTINUOUS INVITRO MULTIPLICATION OF SHOOT BUDS OF NORWAY SPRUCE (PICEA-ABIES L) BY INTERMITTENT APPLICATION OF GROWTH-REGULATORS, Biologia plantarum, 35(1), 1993, pp. 11-15
Citations number
NO
Categorie Soggetti
Plant Sciences
Journal title
Biologia plantarum → ACNP
ISSN journal
00063134
Volume
35
Issue
1
Year of publication
1993
Pages
11 - 15
Database
ISI
SICI code
0006-3134(1993)35:1<11:CIMOSB>2.0.ZU;2-7
Abstract
A culture system was developed which allows continuous production of a dventitious buds. Segments of seedlings germinated on media supplement ed with different growth regulator combinations were used as explants. Cultivation was carried out in two phases, which alternate permanentl y: (I) without and (II) with growth regulators. Thus it was possible t o establish meristematic tissue cultures from 5 - 10 % of the seedling s tested. They have produced buds now for over 3 years with multiplica tion rates of about 1.5 within 5 - 6 weeks.