THE EXTRACELLULAR ACIDIC AND BASIC VIRUS-ELICITED PROTEINS OF CUCUMBER COTYLEDONS

Ten major host-encoded pathogenesis-associated proteins have been foun d extracts of intercellular sap of cucumber cotyledons infected with t obacco necrosis virus. By native and two-dimensional SDS-polyacrylamid e gel electrophoresis, five major acidic virus-elicited proteins have been identified and classified on the basis of their molecular mass in three groups: group 1 included one protein of 14 kD; group 2, one pro tein of 28 kD and group 3, three proteins of 37-40 kD. Another five vi rus-elicited proteins were basic, and were again classified in three g roups: group 1, included two proteins of 15-16 kD; two proteins of 22- 23 kD; group 3, one protein of 40 kD.